| HPV16 E7

HPV-16 E7 encodes a small, 98-aa multifunctional protein. The N-terminal portion of HPV-16 E7 shares amino acid sequence similarity to a portion of conserved regions (CR) 1 and 2 of the adenovirus E1A protein and related sequences in polyomavirus large tumor antigens.HPV-16 E7 are at least in part independent of pocket protein inactivation. In addition, the ability of HPV-16 E7 to induce centrosome-associated mitotic abnormalities is also independent of pocket protein inactivation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	2.65	1.92	1.38	0.95	0.61	0.39	0.23	0.08
Corrected OD	2.57	1.84	1.3	0.87	0.53	0.31	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human HPV16 E7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	95-108	104

 

| Linearity

Samples were spiked with high concentrations of Human HPV16 E7 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	89-96	89-110
	Average (%)	91	94	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108